Journal: Cellular and molecular neurobiology

Article Title: Glucocorticoid-Dependent Retinal Degeneration and Vision Impairment in Mice Susceptible to Prenatal Stress-Induced Behavioral Abnormalities.

doi: 10.1007/s10571-024-01525-x

Figure Lengend Snippet: Fig. 3 Prenatal CUMS impairs retinal neurogenesis and synap- togenesis in susceptible mice. A Retinal sections were immu- nostained for DCX (green) and NeuN (red), and counterstained with DAPI (blue). The ratio of DCX-positive cells to NeuN- positive cells was greater in the susceptible group. Scale bars, 100 μm (magnification, × 200). N = 5. B Retinal sections were immunostained for Thy1 (green) and counterstained for DAPI (blue). The intensi- ties of Thy1 were lower in the susceptible group compared to the control and the resilient groups. Scale bars, 100 μm (magnification, × 200). N = 5. C Eye sections containing retina were immunostained for vGLUT1 (green) and PSD95 (red), and counterstained with DAPI (blue). Pearson’s cor- relation coefficient between vGLUT1 and PSD95 staining was calculated as a measure of colocalization. Colocalization between vGLUT1 and PSD95 was reduced in susceptible group mice. Scale bars, 100 μm (magnification, × 200). N = 5. D Evaluation of retina ganglion cell ultrastructure by scanning electron microscopy revealed a significant reduction in spine intersections in the susceptible group. N = 6 per group. All immunofluorescence images are representative. Quantitative data are presented as mean ± S.E.M. Mann–Whitney U test was conducted. *,** indicates p < 0.05, p < 0.01 versus control group, respectively. ### indicates p < 0.001 versus resilient group

Article Snippet: Antibodies against Thy1 and β-actin were purchased from Santa Cruz Biotechnology (Paso Robles, CA, USA).

Techniques: Control, Staining, Electron Microscopy, Immunofluorescence, MANN-WHITNEY

Journal: Stem Cell Reviews and Reports

Article Title: MicroRNA Cargo in Wharton’s Jelly MSC Small Extracellular Vesicles: Key Functionality to In Vitro Prevention and Treatment of Premature White Matter Injury

doi: 10.1007/s12015-023-10595-1

Figure Lengend Snippet: Characterization of Wharton’s jelly mesenchymal stromal cells (WJ-MSC) of three different donors. (a) WJ-MSC grow adherent on cell culture flasks confirmed by bright-field microscopy. (b) Immunocytochemistry revealed that the WJ-MSC were positive for the MSC markers 5’-nucleotidase (CD73) and Thy-1 (CD90). There was no signal for the hematopoietic marker CD45. (c) FACS analysis of the WJ-MSC showed the expression of MSC markers CD105, CD90, and CD73 in the samples. A broad spectrum of hematopoietic and lymphatic markers, such as CD45, CD34, HLA-DR, CD19, and CD14 were not present in the WJ-MSC samples

Article Snippet: The cells were stained with an adenomatous polyposis coli protein-conjugated (APC) mouse monoclonal antibody against CD105 (562408, BD Biosciences Inc.) and FITC-conjugated mouse monoclonal antibodies against CD90 (SM1170F, OriGene Technologies, Inc., Rockville, MD, USA), CD45 (555482, BD Biosciences Inc.), CD34 (555821, BD Biosciences Inc.), CD14 (MAB1219F, Merck KGaA), and human leukocyte antigen–antigen D related (HLA-DR) (555811, BD Biosciences Inc.), as well as the unconjugated antibodies against CD73 (550256, BD Biosciences Inc.) and CD19 (FCMAB184F, clone HD37, Merck KGaA).

Techniques: Cell Culture, Microscopy, Immunocytochemistry, Marker, Expressing

Journal: Biomedicines

Article Title: A Mitochondrion-Targeting Protein (B2) Primes ROS/Nrf2-Mediated Stress Signals, Triggering Apoptosis and Necroptosis in Lung Cancer

doi: 10.3390/biomedicines11010186

Figure Lengend Snippet: B2-mediated oxidative stress in solid tumors can reduce the expression of tumor markers napsin, Thy1, and CD133 in NOD/SCID mice. ( A ) Solid tumors obtained from the lungs of NOD/SCID mice that underwent treatment with 5-FU (positive control), vehicle (negative control), Flag-B2, Flag, and Flag-ΔB2 (mutant control) were immunostained with anti-napsin, anti-Thy1, and anti-CD133 antibodies. Scale bar = 100 uM. Quantification of signal intensity of ( B ) napsin, ( C ) Thy1, and ( D ) CD133 (All N = 3). The data are representative of three separate experiments, and the error bars represent the SEMs. * p < 0.05 and ** p < 0.01, as analyzed by one-way ANOVA with Tukey’s multiple comparisons.

Article Snippet: Antibodies against Napsin (Cell Signaling Technology), CD133 (Cell Signaling Technology), and Thy1 (D3V8A) (Cell Signaling Technology) were used.

Techniques: Expressing, Positive Control, Negative Control, Mutagenesis, Control

Journal: Biomedicines

Article Title: A Mitochondrion-Targeting Protein (B2) Primes ROS/Nrf2-Mediated Stress Signals, Triggering Apoptosis and Necroptosis in Lung Cancer

doi: 10.3390/biomedicines11010186

Figure Lengend Snippet: Hypothesized effect of mitochondrion-targeting protein B2 on multiple signaling pathways by ROS-mediated oxidative stress in cancer cells. The expression of the B2 gene in A549 lung tumor cells or in A549 cell-induced solid tumors in NOD/SCID mice induced ROS-mediated stress signals, causing p53/Bax-mediated apoptosis and RIPK3-mediated necroptosis. The B2 protein can transfer into the mitochondria using its novel transferring signal sequence. 41 RTFVISAHAA 50 . It then regulates complex II activity, suppressing ATP production and enhancing ROS generation, thereby repressing cancer cell growth. B2 triggers ROS/Nrf2-mediated stress, further affecting multiple signaling pathways, such as (1) the induction of the p53- and Bax-mediated apoptotic signals and the RIPK3-mediated necroptosis signal, as well as (2) a reduction in the cancer marker expressions of CD133, Thy1, and napsin in mice, which are responsible for cell migration and invasion in lung tumors.

Article Snippet: Antibodies against Napsin (Cell Signaling Technology), CD133 (Cell Signaling Technology), and Thy1 (D3V8A) (Cell Signaling Technology) were used.

Techniques: Protein-Protein interactions, Expressing, Transferring, Sequencing, Activity Assay, Marker, Migration

Journal: Cell Communication and Signaling : CCS

Article Title: SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression

doi: 10.1186/s12964-022-00921-4

Figure Lengend Snippet: LR-MSCs-myofibroblast transdifferentiation in patients with IPF. A Representative results of H&E staining and Masson staining in normal lung and lungs from patients with IPF. B Representative images of dual staining for CD90 (green) and α-SMA (red). White arrow indicates LR-MSCs. Quantification is shown in the right panel. * P < 0.05, IPF group vs. control group. Scale bar, 50 μm

Article Snippet: The slides were then placed in a microwave oven until the antigen retrieval solution reached 100 °C for 10 min one and for 2 min four times, cooled to room temperature for 20 min and washed with PBS for 5 min. Primary antibodies against CD90 (Cell Signaling, #13,801, 1:200; Abcam, Ab181469, 1:200), SENP1 (Abcam, Ab236094, 1:200), and alpha-smooth muscle actin (α-SMA) (Abcam, Ab240654, 1:200) were incubated with the slides overnight at 4 °C.

Techniques: Staining

Journal: Cell Communication and Signaling : CCS

Article Title: SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression

doi: 10.1186/s12964-022-00921-4

Figure Lengend Snippet: SENP1 mediated deSUMOylation increased in LR-MSCs-myofibroblast of lung tissue from patients with IPF. A Western blotting was applied to detect the levels of SENP1, α-SMA, and Collagen I. Quantification is shown in the lower panel. *P < 0.05, IPF group vs . control group. B Representative images of dual staining for CD90 (green) and SENP1 (red). The white arrow indicates LR-MSCs. Quantification is shown in the right panel. * P < 0.05, IPF group vs. control group. Scale bar, 50 μm. The results are shown as the means ± SD

Article Snippet: The slides were then placed in a microwave oven until the antigen retrieval solution reached 100 °C for 10 min one and for 2 min four times, cooled to room temperature for 20 min and washed with PBS for 5 min. Primary antibodies against CD90 (Cell Signaling, #13,801, 1:200; Abcam, Ab181469, 1:200), SENP1 (Abcam, Ab236094, 1:200), and alpha-smooth muscle actin (α-SMA) (Abcam, Ab240654, 1:200) were incubated with the slides overnight at 4 °C.

Techniques: Western Blot, Staining

Journal: Biomedicines

Article Title: Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

doi: 10.3390/biomedicines9111565

Figure Lengend Snippet: Analysis of the character of fibroblasts. The fibroblasts from stomach tissue (hGFCs) and BJ cells were collected, and the expressions of fibroblast markers calponin and CD90 and epithelium marker VE-cadherin were analyzed by ( A ) RT-PCR. In addition, the protein expressions of hGFCs, BJ cells, and skin fibroblasts were analyzed by Western blot. ( B ) The expression of fibroblast marker CD90 is presented, and none of epithelium marker VE-cadherin. ( C ) The wound healing assay was performed to investigate the migration of fibroblasts. ( D ) The data revealed that cell migration was slow in hGFCs compared with BJ cells and skin fibroblasts. Images were acquired at 40X. The hGFCs reveal less migration activity than skin fibroblasts and BJ cells. The experiment was performed in triplicate, and data are presented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: Then, the samples were run on 10% SDS-PAGE, electroblotted onto membranes, and incubated with primary antibodies against human CD90 (13801, Cell Signaling Technology, Danvers, MA, USA), VE-cadherin (MA5-17050, Thermo Fisher Scientific, Waltham, MA, USA), and GAPDH (PA1-987; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Marker, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Wound Healing Assay, Migration, Activity Assay